Adipocyte protein modification by Krebs cycle intermediates and fumarate ester-derived succination

作者：Manuel Allison M; Frizzell Norma^*

来源：Amino Acids, 2013, 45(5): 1243-1247.

DOI：10.1007/s00726-013-1568-z

• Succination
• S-(2-succino)cysteine
• Succinylation
• Acetylation
• Fumarate
• Ester
• Glutathione

Protein succination, the non-enzymatic modification of cysteine residues by fumarate, is distinguishable from succinylation, an enzymatic reaction forming an amide bond between lysine residues and succinyl-CoA. Treatment of adipocytes with 30 mM glucose significantly increases protein succination with only a small change in succinylation. Protein succination may be significantly increased intracellularly after treatment with fumaric acid esters, however, the ester must be removed by saponification to permit 2SC-antibody detection of the fumarate adduct.

• 出版日期2013-11