A metallothionein-like protein of rice (rgMT) functions in E-coli and its gene expression is induced by abiotic stresses

作者：Jin Shumei; Cheng Yuxiang; Guan Qingjie; Liu Dali; Takano Tetsuo; Liu Shenkui^*

来源：Biotechnology Letters, 2006, 28(21): 1749-1753.

DOI：10.1007/s10529-006-9152-1

• abiotic stress
• gene expression
• glutathione-S-transferase (GST) fusion protein
• metallothionein (MT)

A metallothionein-like (rgMT) gene was isolated from a rice (Oryza sativa L.) root cDNA library that was prepared from plants grown under NaHCO(3) stress. The rgMT gene expression was induced in rice leaves and roots under several abiotic stresses from salts (NaCl and NaHCO(3)), drought (PEG) and metals (CuCl(2,) ZnCl(2), CdCl(2)). The results suggested that the rgMT gene was expressed in response to environmental stresses. The rgMT gene was expressed in Escherichia coli, and the final yield of the purified rgMT protein was 4.8 mg g(-1) dry cells. Tolerance of E. coli expressing GST-rgMT fusion protein to Cu(2+), Zn(2+) and Cd(2+) supercript stopwas enhanced, and cells dry weight increased 0.04 mg, 0.17 mg and 0.07 mg in 1 ml culture treated with either CuCl(2), ZnCl(2) or CdCl(2), respectively, compared with control after 6 h culture.

• 出版日期2006-11
• 单位东北林业大学