Antiplatelet Activity of alpha-Lipoic Acid

作者：Lai Yuan Shu; Shih Ching Yu; Huang Yu Feng; Chou Tz Chong^*

来源：Journal of Agricultural and Food Chemistry, 2010, 58(15): 8596-8603.

DOI：10.1021/jf101518p

• alpha-Lipoic acid
• platelet aggregation
• thromboxane A(2)
• cyclic AMP
• Ca(2+) mobilization

alpha-Lipoic acid (ALA) is often used as a dietary supplement to prevent and treat chronic diseases associated with excessive oxidative stress. The aim of this study was to investigate the mechanisms of the antiplatelet activity of ALA. ALA significantly inhibited collagen-induced platelet aggregation, thromboxane B(2) (TXB(2)) formation, Ca(2+) mobilization, and protein kinase C alpha (PKC alpha) activation, but ALA itself increased cyclic AMP formation in rabbit washed platelets. However, the effects of ALA on the above platelet responses were markedly reversed by the addition of 2'5'-ddAdo, an adenylate cyclase inhibitor. Additionally, increased reactive oxygen species (ROS) formation and cyclooxygenase-1 activity stimulated by arachidonic acid were inhibited by ALA. In conclusion, we demonstrated that ALA possesses an antiplatelet activity, which may be associated with an elevation of cyclic AMP formation, involving subsequent inhibition of TXA(2), Ca(2+) mobilization, and PKC alpha-mediated pathways. Moreover, inhibition of ROS formation and increase of platelet membrane fluidity may also involve its actions.

• 出版日期2010-8-11