Different shades of cholesterol: Gold nanoparticles supported on MOS2 nanoribbons for enhanced colorimetric sensing of free cholesterol

作者：Nirala Narsingh R; Pandey Shobhit; Bansal Anushka; Singh Vijay K; Mukherjee Bratindranath; Saxena Preeti S^*; Srivastava Anchal

来源：Biosensors and Bioelectronics, 2015, 74: 207-213.

DOI：10.1016/j.bios.2015.06.043

• Molybdenum disulfide nanoribons
• Gold nanoparticles
• Cholesterol
• Colorimetric
• Peroxidase mimetic
• Color wheel

In the present study, we manifest that traditionally used gold nanoparticles when supported on molybdenum disulfide nanoribbons matrix (MoS2 NRs-Au NPs) show synergistically enhanced intrinsic peroxidase like catalytic activity and can catalyze the oxidation of 3,3',5,5' tetramethyl benzidine by H2O2 to produce a highly sensitive blue shade product depending on level of free cholesterol, when tested on complex system of human serum. Further the system attests appreciable kinetics, owing to K-m value as low as 0.015 mM and better loading capacity (V-max = 6.7 x 10(-6) M s(-1)). Additionally, the proposed system is stable for weeks with ability to perform appreciably in wide pH (3-6) and temperature range (25-60 degrees C). Utilizing this potential, the present work proposes a cholesterol detection color wheel which is used along with cost effective cholesterol detection strips fabricated out of proposed MoS2 NRs-Au NPs system for quick and reliable detection of free cholesterol using unaided eye.

• 出版日期2015-12-15