Mast cells cultured from IL-3-treated mice show impaired responses to bacterial antigen stimulation

作者：Vukman Krisztina V; Visnovitz Tamas; Adams Paul N; Metz Martin; Maurer Marcus; O' Neill Sandra M

来源：Inflammation Research, 2012, 61(1): 79-85.

DOI：10.1007/s00011-011-0394-6

• Peritoneal mast cells
• Interleukin-3
• Ex-vivo culture
• C57BL/6 mice

Objective and design This study exploits the biological activity of interleukin (IL)-3 to generate high yields of peritoneal mast cells ex vivo in order to examine proinflammatory immune responses in ex-vivo culture. %26lt;br%26gt;Material or subjects Mast cells were obtained from the peritoneal cavity of C57BL/6 mice. Treatment Mice were injected intraperitoneally twice per day for 5 days with IL-3 (40-50 mu g/ml) to increase mast cell numbers. %26lt;br%26gt;Methods Histological studies examined mast cell numbers in the peritoneal cavity, intestine, lung, spleen and skeletal muscle. Peritoneal mast cells cultured ex vivo (PCMCs) were stimulated for 24 h with lipopolysaccharide and Bordetella pertussis antigen and secretion of tumour necrosis factor-a, IL-6, IL-4, IL-5, IL-10 and interferon-c into supernatant was measured by commercial ELISA. Cell surface marker expression of Fc epsilon RI, c-kit, OX40L and TLR2 was measured by flow cytometry. Mast cell degranulation was measured using a beta-hexosaminidase assay. %26lt;br%26gt;Results IL-3 treatment increases mast cell numbers in the peritoneal cavity, spleen and muscle but not intestine and lung of C57BL/6 mice. PCMCs generated from IL-3-treated mice exhibit impaired growth, differentiation and responses to activation as measured by decreased cytokine secretion and cell surface marker expression. %26lt;br%26gt;Conclusion Mast cells cultured from IL-3-treated mice show impaired responses.

• 出版日期2012-1