Differences in AMPA and GABA(A/B) receptor subunit expression between the chronically reorganized cortex and brainstem of adult squirrel monkeys

作者：Mowery Todd M^*; Sarin Rohini M; Kostylev Polina V; Garraghty Preston E

来源：Brain Research, 2015, 1611: 44-55.

DOI：10.1016/j.brainres.2015.03.010

• Reorganization
• Peripheral nerve injury
• Long-term recovery
• GABA
• AMPA
• Area 3b cortex
• Cuneate nucleus brainstem
• Non-human primate

The primate somatosensory neuraxis provides a highly translational model system with which to investigate adult neural plasticity. Here, we report immunohistochemical staining data for AMPA and GABA(A/B) receptor subunits of area 3b cortex and cuneate nucleus of adult squirrel monkeys one to five years after median and ulnar nerve transection. In Area 3B cortex, the expression of GluR1 AMPAR subunits in reorganized regions are significantly increased, while the expression of GluR2/3 AMPAR subunits are not. GABA(A) alpha 1 subunit expression in the reorganized region is not significantly different from control regions. Presynaptic GABA(B)R1a subunit expression was also not significantly different between reorganized and control regions, while postsynaptic GABA(B)R1b subunit expression was significantly decreased. In the cuneate nucleus of the brainstem, the expression of GluR1 AMPAR subunits in reorganized regions was not significantly different, while GluR2/3 AMPAR subunit expression was significantly elevated. GABA(A) alpha 1 subunit expression in the reorganized region was significantly decreased. Presynaptic GABA(B)R1a subunit expression was not significantly different, while postsynaptic GABA(B)R1b subunit expression was significantly decreased. When subunit expression is compared, brainstem and cortical patterns diverge over longer periods of recovery. Persistent patterns of change in the cortex are stable by 1-year. Alternatively, subunit expression in the cuneate nucleus one to five years after nerve injury is similar to that seen 1-month after a reorganizing injury. This suggests that cortical plasticity continues to change over many months as receptive field reorganization occurs, while brainstem plasticity obtains a level of stable persistence by one month. Published by Elsevier B.V.

• 出版日期2015-6-22