Antitumor immunity by a dendritic cell vaccine encoding secondary lymphoid chemokine and tumor lysate on murine prostate cancer

作者：Lu, Jun^*; Zhang, Qi; Liang, Chun Min; Xia, Shu Jie; Zhong, Cui Ping; Wang, Da Wei

来源：Asian Journal of Andrology, 2008, 10(6): 883-889.

DOI：10.1111/j.1745-7262.2008.00431.x

• dendritic cell
• secondary lymphoid chemokine
• prostate cancer
• tumor lysate

Aim: To investigate the antitumor immunity by a dendritic cell (DC) vaccine encoding secondary lymphoid chemokine gene and tumor lysate on murine prostate cancer. Methods: DC from bone marrow of C57BL/6 were transfected with a plasmid vector expressing secondary lymphoid chemokine (SLC) cDNA by Lipofectamine2000 liposome and tumor lysate. Total RNA extracted from SLC lysate-DC was used to verify the expression of SLC by reverse transcriptase-polymerase chain reaction (RT-PCR). The immunotherapeutic effect of DC vaccine on murine prostate cancer was assessed. Results: We found that in the prostate tumor model of C57BL/6 mice, the adminstration of SLC lysate-DC inhibited tumor growth most significantly when compared with SLC-DC, lysate-DC, DC or phosphate buffer solution (PBS) counterparts (P < 0.01). Immunohistochemical fluorescent staining analysis showed the infiltration of more CD4( ), CD8( ) T cell and CD11c( ) DC within established tumor treated by SLC lysate-DC vaccine than other DC vaccines (P < 0.01). Conclusion: DC vaccine encoding secondary lymphoid chemokine and tumor lysate can elicit significant antitumor immunity by infiltration of CD4( ), CD8( ) T cell and DC, which might provide a potential immunotherapy method for prostate cancer.

• 出版日期2008-11-1
• 单位复旦大学; 上海交通大学