科研之友
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摘要
Single nucleotide polymorphisms ( SNPs) can contribute to genetic predispositions or serve as genetic markers that are associated with complex diseases. So far, a few SNP arrays containing a limited number of SNPs have been used in routine genetic testing. This study described an oligochip- based method that genotypes two SNPs (- 511 and - 31) in the promoter region of the interleukin ( IL)-1 beta gene. The sensitivity of this SNP genotyping method is derived from polymerase chain reaction ( PCR)- amplified allele- specific primer- probes with a biotin label incorporated from the reverse primers. The amplified primer- probes can specifically hybridize with the oligonucleotides that are spotted on the oligochip. This oligochip- based method successfully discriminated the two biallelic SNPs with 9 different genotypes and all the genotyping results are in concordance with those from PCR restriction fragment length polymorphism (RFLP) analysis. Selective samples with various genotypes were also confirmed by direct sequencing. This method was applied in the genotyping of the patients with tuberculosis or gastric cancer and healthy controls. In the case control study, our genotyping data supported the reported association between gastric cancer and the genotypes of IL-1 beta-31 TT and -511 CC (p < 0.05). We also found that there is a significant difference of IL-1 beta-31 genotypes between 98 tuberculosis patients and healthy controls (p < 0.002). All of our results demonstrated that the oligochip can effectively and accurately identify SNP genotypes in the IL- 1 beta promoter region.
- 出版日期2007-9
- 单位山东师范大学; 济南市中心医院
