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摘要
A simple and sensitive method of gas chromatography-mass spectrometry for quantification of beta-elemene in human plasma was developed and validated. An n-hexane-based liquid-liquid extraction assisted by saturated sodium chloride solution was used for sample preparation. Naphthalene was chosen as the internal standard. The calibration curve (r (2) = 0.9997) of beta-elemene was established at the range of 0.0655-40.00 mu g mL(-1) and the lower limit of quantification (LLOQ) was 0.0655 mu g mL(-1) using 200 mu L of plasma, respectively. The accuracy was in a range of 87.1-101.7 %, and intra- and inter-day precisions (RSD %) at three QC levels were all less than 10 %. The LLOQ of 0.0665 mu g mL(-1) is sensitive enough to perform pharmacokinetic studies after beta-elemene administration.
