科研之友
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摘要
Adipose tissue-derived Stem Cells(ADSCs) are utilized as a useful resource in many cell-based therapies The ADSCs are often cryopreserved for future use but some cryoprotectants may have harmful influence on the stem cell quality such as sternness or viability In this study, we investigated the influence of cryoprotectants such as DMSO, FBS and disaccharides on cell quality ADSCs were frozen with variable combinations of cryoprotectants The cells were stored in liquid nitrogen for a given period. The cells were thawed and tested for viability with trypan blue, the proportion of ADSCs were determined by flow cytometry analysis and the multipotency of the cells was assessed by differentiation potential of the cells into osteocytes or adipocytes. In the preliminary study, FBS had no additional benefit on the cells and, thus, the use of cryopreservation solution with serum free and low concentration of DMSO(5%) is suitable for cryopreservation of ADSCs used for cell-based therapy From these data, we studied cell viability, MSC phenotype and differentiation potential as parameter of stability assessment In result, there was no significant changes of viability, phenotype and representative CD marker according to our freezing condition/duration and concentration or volume of final products
- 出版日期2010-8
