Fe3O4@PAM@ NTA-Ni2+ Magnetic Composite Nanoparticles for Highly Specific Separation of His-tagged Proteins

作者：Guo Huiling^*; Sun Honghao; Su Zhengding; Hu Shuchao; Wang Xiaotao

来源：Journal of Wuhan University of Technology-Materials Science Edition, 2018, 33(3): 559-565.

DOI：10.1007/s11595-018-1860-6

• magnetic nanoparticles
• polyacrylamide
• protein separation
• his-tagged protein

A facile approach has been developed to synthesize Fe3O4@PAM (polyacrylamide) nanoparticles (NPs) with carboxyl groups on the surfaces by copolymerization with acrylamide and acrylic acid in Fe3O4 NPs aqueous suspension. Nitrilotriacetic acid (NTA) was conjugated to the magnetic NPs via well-known carboniimide chemistry using EDC and NHS. The Ni2+ ions loaded on the surface of NPs provide abundant docking sites for immobilization of His-tagged green fluorescent proteins (His-tagged GFP). The high magnetic property of Fe3O4@PAM@NTA-Ni2+ allows an easy separation of the NPs from solution under an external magnetic field, with high His-tagged protein binding capacity (42 mu g protein/mg of NPs). The NPs can be recycled for at least four times without significant loss of binding capacity to proteins. These materials show great potential to separate His-tagged protein with low-cost purification at industrial scale.

• 出版日期2018-6
• 单位湖北工业大学