Association between Long Interspersed Nuclear Element-1 Methylation and Relative Telomere Length in Wilms Tumor

作者：Chang, Hui-Bo; Zou, Ji-Zhen; He, Cai; Zeng, Rui; Li, Yuan-Yuan; Ma, Fei-Fei; Liu, Zhuo; Ye, Hui; Wu, Jian-Xin^*

来源：Chinese Medical Journal, 2015, 128(22): 3055-3061.

DOI：10.4103/0366-6999.169071

• 5-aza-2'-deoxycytidine
• Hypomethylation
• Long Interspersed Nuclear Element-1
• Relative Telomere Length
• Wilms Tumor

Background: DNA hypomethylation of long interspersed nuclear elements-1 (LINEs-1) occurs during carcinogenesis, whereas information addressing LINE-1 methylation in Wilms tumor (WT) is limited. The main purpose of our study was to quantify LINE-1 methylation levels and evaluate their relationship with relative telomere length (TL) in WT. @@@ Methods: We investigated LINE-1 methylation and relative TL using bisulfite-polymerase chain reaction (PCR) pyrosequencing and quantitative PCR, respectively, in 20 WT tissues, 10 normal kidney tissues and a WT cell line. Significant changes were analyzed by t-tests. @@@ Results: LINE-1 methylation levels were significantly lower (P < 0.05) and relative TLs were significantly shorter (P < 0.05) in WT compared with normal kidney. There was a significant positive relationship between LINE-1 methylation and relative TL in WT (r = 0.671, P = 0.001). LINE-1 Methylation levels were significantly associated with global DNA methylation (r = 0.332, P < 0.01). In addition, relative TL was shortened and LINE-1 methylation was decreased in a WT cell line treated with the hypomethylating agent 5-aza-2'-deoxycytidine compared with untreated WT cell line. @@@ Conclusion: These results suggest that LINE-1 hypomethylation is common and may be linked to telomere shortening in WT.

• 出版日期2015-11-20
• 单位首都儿科研究所