A reliable, single-step method for gender determination in black rhinoceros from low-copy template DNA

作者：Cain Bradley^*; Watts Phillip C; Wandera Antony B; Stevens Wood Barry; Shawcross Susan G

来源：Conservation Genetics Resources, 2013, 5(3): 655-657.

DOI：10.1007/s12686-013-9875-y

• Black rhinoceros
• Gender determination
• Non-invasive sampling
• 5 %26apos
• exonuclease assay
• ZFX/ZFY

We describe an accurate, single-step 5%26apos;exonuclease assay for gender determination of black rhinoceros Diceros bicornis, which targets a single nucleotide polymorphism in exon 4 of the sex-linked zinc finger homologues in this species. This simple, high-throughput method facilitates the multiple-tubes approach that is necessary when using genetic material derived from non-invasive samples such as faecal material.

• 出版日期2013-9