科研之友
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摘要
In this study, chemical composition and antioxidant properties of Ocimum gratissimum (Og) were investigated. Leaves of Og were extracted using ethanol to obtain an ethanolic crude extract (ECE), and then ECE was sequentially partitioned with petroleum ether, ethyl acetate, n-butanol, and deionized water to give the corresponding petroleum ether fraction, ethyl acetate fraction (EAF), n-butanol fraction, and water fraction. Total phenolic contents of ECE and its fractions were estimated using Folin-Ciocalteu reagent, and total flavonoid contents were assessed using a colorimetric assay. Antioxidant activity was assessed using ferric reducing antioxidant power assay, total antioxidant capacity assay, and 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) free radical scavenging assays. All samples of Og exhibited activity in all in vitro antioxidant assays, and EAF exhibited the highest activity among the tested samples. By further purification procedure, four compounds were isolated from EAF. They were identified as: beta-sitosterol (1), ursolic acid (2), salvigenin (3), and trans-ferulic acid (4) by analyses of NMR and MS spectral data. EAF is rich in antioxidants that can be used as an effective preservative in food industry.
