A conveniently prepared and hypersensitized small molecular fluorescent probe: Rapidly detecting free zinc ion in HepG2 cells and Arabidopsis

作者：Gan, Xiaoping; Sun, Ping; Li, Hong; Tian, Xiaohe; Zhang, Baowei^*; Wu, Jieying; Tian, Yupeng; Zhou, Hongping^*

来源：Biosensors and Bioelectronics, 2016, 86: 466-471.

DOI：10.1016/j.bios.2016.06.087

• Fluorescence probes
• Zinc ion
• Rapid-response ability
• High sensitivity
• Bio-imaging application

In this paper, we reported a conveniently prepared fluorescent probe for zinc ions detection, which constructed by the condensation reaction between p-(benzothiazolyl)aniline with 4, 4- diethylaminesalicylaldehyde. The sensing ability of the probe toward zinc ions in vitro was tested by a series of UV-Vis and fluorescence spectroscopy studies, which showed that the probe possessed high sensitivity with a detection limit of 5.8 nM and a rapid response time of 10 s. We also carried out fluorescent bio-imaging of the probe for zinc ions in human liver hepatocellular carcinoma cells (HepG2), which showed that the probe could be utilized to detect the intracellular endogenous zinc ions visually without introducing external zinc sources. Meanwhile, co-staining experiment with organelle selective trackers was performed to illustrate that the probe could locate at endoplasmic reticulum. Finally, we successfully used it as a zinc ion developer in plant tissue, which clearly demonstrated the distribution of zinc ions in the growth stage of plant tissue.

• 出版日期2016-12-15
• 单位安徽大学; 安徽农业大学