Development of a Two-Step Multiplex PCR Assay for Typing of Capsular Polysaccharide Synthesis Gene Clusters of Streptococcus suis

作者：Okura Masatoshi; lachance ClauDe; Osaki Makoto; Sekizaki Tsutomu; Maruyama Fumito; Nozawa Takashi; Nakagawa Ichiro; Hamada Shigeyuki; Rossignol Celine; Gottschalk Marcelo; Takamatsu Daisuke^*

来源：Journal of Clinical Microbiology, 2014, 52(5): 1714-1719.

DOI：10.1128/JCM.03411-13

We developed a practical and easy two-step multiplex PCR assay to aid in serotyping of Streptococcus suis. The assay accurately typed almost all of the serotype reference strains and field isolates of various serotypes and also identified the genotypes of capsular polysaccharide synthesis gene clusters of some serologically nontypeable strains.

• 出版日期2014-5