Asiasari sieboldii suppresses inflammatory mediators through the induction of hemeoxygenase-1 expression in RAW264.7 cells

作者：Lee Jin Ah; Lee Mee Young; Seo Chang Seob; Ha Hyekyung; Lee Hoyoung; Kim Jung Hoon; Shin Hyeun Kyoo^*

来源：Immunopharmacology and Immunotoxicology, 2012, 34(1): 15-20.

DOI：10.3109/08923973.2011.572261

• Asiasari sieboldii
• anti-inflammatory
• hemeoxygenase-1 (HO-1)

Objective: Asiasari sieboldii is widely used in Korean traditional medicine. In this study, we examined the anti-inflammatory effects of A. sieboldii ethanolic extract (ASEE) in a lipopolysaccharide (LPS)-induced murine macrophage RAW264.7 cell, and then sought to understand the mechanism(s) underlying the observed effects. %26lt;br%26gt;Materials and methods: The production levels of nitrite oxide (NO), prostaglandin E-2 (PGE(2)) and cytokines were measured using the Griess reagent and enzyme-linked immunosorbent assays (ELISA), while the cell protein expression levels were monitored by Western blot analysis. %26lt;br%26gt;Results: Our results revealed that ASEE had prominent inhibitory effects on NO, PGE(2), interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha production, as well as the expression of inducible nitrite oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), in LPS-induced RAW264.7 cells. Mechanistically, ASEE upregulated the expression of hemeoxygenase-1 (HO-1), and inhibited the nuclear translocation of nuclear factor (NF)-kappa B by preventing degradation of inhibitor kappa B-alpha (I kappa B-alpha). %26lt;br%26gt;Conclusion: These results indicate that the anti-inflammatory activity of ASEE occurs at least partially through the induction of HO-1 and subsequent suppression of the NF-kappa B pathway.

• 出版日期2012-2
• 单位河北医科大学