A simple and inexpensive particle agglutination test to distinguish recent from established HIV-1 infection

作者：Hong Li; Ketema Fassil; Sill Anne M; Kreisel Kristen M; Cleghorn Farley R; Constantine Niel T^*

来源：International Journal of Infectious Diseases, 2007, 11(5): 459-465.

DOI：10.1016/j.ijid.2006.12.010

• sensitive/less sensitive
• detuned
• HIV
• incidence
• particle agglutination
• surveillance

Objectives: We sought to modify the Serodia HIV-1/HIV-2 particle agglutination assay (PA), a simple and cost-effective HIV assay that is used globally for the detection of HIV antibodies, as a sensitive/less sensitive test (S/LS) to identify recently infected individuals and to estimate HIV incidence.
Methods: The Serodia PA test was modified as an S/LS test (PA-LS) by using HIV antigen-coated gelatin particles at a dilution of 1:68 and a specific diluent, and calibrated using 37 HIV clade B seroconversion panels (309 samples) from Trinidad and from a commercial source that were tested at dilution intervals from 1:10 to 1:80 000. The greatest sensitivity for correctly classifying samples from recent and established infections was determined by receiver operator curve (ROC) analysis.
Results: At a 1:40 000 sample dilution and a days post-seroconversion cutoff of 190 days, the PALS test yielded a 97% sensitivity for classifying recent and established infection samples. Furthermore, at a 1:20 000 dilution, the positive predictive value for correctly identifying recently infected individuals was 99%. The PA-LS test offers a 30-44-fotd cost saving over currently available S/LS tests.
Conclusion: A modified, low cost and simple-to-perform PA test is appropriate for use in resource-limited countries, and has exhibited excellence in distinguishing recent from established HIV infection.

• 出版日期2007-9
• 单位云南省疾病预防控制中心