Prometryne-induced oxidative stress and impact on antioxidant enzymes in wheat

作者：Jiang Lei; Yang Hong^*

来源：Ecotoxicology and Environmental Safety, 2009, 72(6): 1687-1693.

DOI：10.1016/j.ecoenv.2009.04.025

• Ecotoxicological effect
• Antioxidant enzyme
• Prometryne
• Herbicide stress
• Wheat (Triticum aestivum)

Prometryne is one of the herbicides widely used for controlling weed/grass in agricultural practice. However, whether it has an adverse effect on crops is unknown. In this study, we investigated prometryne-induced oxidative stress in wheat (Triticum aestivum). Wheat plants were grown in soils with prometryne at 0-24 mg kg(-1) soil. The growth of wheat treated with prometryne was inhibited. Chlorophyll content significantly decreased even at the low level of prometryne (4 mg kg(-1) soil). Accumulation of thiobarbituric acid reactive substances (TBARS), an indicator of cellular peroxidation, increased, suggesting oxidative damage to the plants. The prometryne-induced oxidative stress triggered significant changes in activities of a variety of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX) and glutathione S-transferase (GST). Activities of the enzymes showed a general increase at low prometryne concentrations but a decrease at high levels. Analysis of non-denaturing polyacrylamide gel electrophoresis (PAGE) confirmed the results. To get an insight into the molecular response, a qRT-PCR-based assay was performed to analyze the transcript abundance of Cu/Zn-SOD and GST with prometryne exposure. Our analysis revealed that both genes displayed up-regulated expression patterns similar to the activities of the two enzymes. These data imply that prometryne-induced oxidative stress was responsible for the disturbance of the growth and antioxidant defensive systems in wheat plants.

• 出版日期2009-9
• 单位南京农业大学