A population survey of Beauveria bassiana in the microhabitat of the red turpentine beetle, Dendroctonus valens, in Chinese pine forests

作者：Yao, Jian; Zhang, Longwa; Chen, Xuejiao; Li, Zengzhi^*

来源：Biocontrol Science and Technology, 2012, 22(11): 1254-1267.

DOI：10.1080/09583157.2012.724382

• Beauveria bassiana
• Dendroctonus valens
• population survey
• substrate

Field survey of the entomopathogenic fungus Beauveria bassiana in association with the red turpentine beetle, Dendroctonus valens, was undertaken in three pine plantations in Northern China. In total, 88 strains of B. bassiana sensu lato were isolated from the soil, bark, beetle frass, living adult and cadaver samples and soil was proved to be an important inoculum reservoir for fungal entomopathogens. Of these, 77 isolates were included for genetic diversity analysis by PCR for inter-simple sequence repeats (ISSR). Genetic diversity and population structure analysis of the isolates from three sites and five niches demonstrated high genetic diversity and heterogeneity between and/or within populations. Wright's statistics revealed a high gene flow rate (4.529) among the three populations, especially among the soil-derived isolate subpopulations. Low variation was mainly caused (94.8%) by variation among different substrates, suggesting the importance of microhabitat substrates on genetic diversity of B. bassiana. Phylogenetic variation was not associated with geographic distance.

• 单位
  安徽农业大学