Determination of triamcinolone acetonide in silicone oil and aqueous humor of vitrectomized rabbits%26apos; eyes: Application for a pharmacokinetic study with intravitreal triamcinolone acetonide injections (Kenalog (R) 40)

作者：Fernandes Cunha Gabriella M^*; Saliba Juliana B; Siqueira Rubens C; Jorge Rodrigo; Silva Cunha Armando

来源：Journal of Pharmaceutical and Biomedical Analysis, 2014, 89: 24-27.

DOI：10.1016/j.jpba.2013.10.025

• Silicone oil
• Triamcinolone acetonide
• Intravitreal injections
• Vitrectomy
• HPLC-UV

A simple and accurate method including liquid-liquid extraction and protein precipitation procedures from silicone oil and aqueous humor samples followed by high-performance liquid chromatography (HPLC-UV) was developed and validated to determine the pharmacokinetic profile of triamcinolone acetonide in silicone oil and aqueous humor of rabbits%26apos; eyes submitted to the pars plana vitrectomy surgery. The method was successfully applied to quantify the drug remaining in silicone oil and aqueous humor (LOQ range of 1 mu g/mL). The triamcinolone acetonide remained in silicone oil and aqueous humor of vitrectomized rabbits%26apos; eyes for four weeks after the intravitreal injections.

• 出版日期2014-2-15