Asymmetric spindle pole formation in CPAP-depleted mitotic cells

作者：Lee Miseon; Chang Jaerak; Chang Sunghoe; Lee Kyung S; Rhee Kunsoo^*

来源：Biochemical and Biophysical Research Communications, 2014, 444(4): 644-650.

DOI：10.1016/j.bbrc.2014.01.129

• Spindle pole
• CPAP
• Centriole maturation
• Asymmetric cell division

CPAP is an essential component for centriole formation. Here, we report that CPAP is also critical for symmetric spindle pole formation during mitosis. We observed that pericentriolar material between the mitotic spindle poles were asymmetrically distributed in CPAP-depleted cells even with intact numbers of centrioles. The length of procentrioles was slightly reduced by CPAP depletion, but the length of mother centrioles was not affected. Surprisingly, the young mother centrioles of the CPAP-depleted cells are not fully matured, as evidenced by the absence of distal and subdistal appendage proteins. We propose that the selective absence of centriolar appendages at the young mother centrioles may be responsible for asymmetric spindle pole formation in CPAP-depleted cells. Our results suggest that the neural stem cells with CPAP mutations might form asymmetric spindle poles, which results in premature initiation of differentiation.

• 出版日期2014-2-21
• 单位NIH