A model system for targeted drug release triggered by biomolecular signals logically processed through enzyme logic networks

作者：Mailloux Shay; Halamek Jan; Katz Evgeny^*

来源：Analyst, 2014, 139(5): 982-986.

DOI：10.1039/c3an02162a

A new Sense-and-Act system was realized by the integration of a biocomputing system, performing analytical processes, with a signal responsive electrode. A drug-mimicking release process was triggered by biomolecular signals processed by different logic networks, including three concatenated AND logic gates or a 3-input OR logic gate. Biocatalytically produced NADH, controlled by various combinations of input signals, was used to activate the electrochemical system. A biocatalytic electrode associated with signal-processing %26quot;biocomputing%26quot; systems was electrically connected to another electrode coated with a polymer film, which was dissolved upon the formation of negative potential releasing entrapped drug-mimicking species, an enzyme-antibody conjugate, operating as a model for targeted immune-delivery and consequent %26quot;prodrug%26quot; activation. The system offers great versatility for future applications in controlled drug release and personalized medicine.

• 出版日期2014