New HPLC Method for Quality Control of beta-Escin in Aesculus hippocastanum L. Hydroalcoholic Extract

作者：De Almeida Priscila A; Alves Michele C; Polonini Hudson C; Dutra Lidiane S; Leite Magda N; Raposo Nadia R B; Ferreira Anderson de O; Brandao Marcos A F^*

来源：Latin American Journal of Pharmacy, 2013, 32(7): 1082-1087.

• Aesculus hippocastanum
• High pressure liquid chromatography
• Quality control

The major phytoconstituent present in Aesculus hippocastanum L. is a complex mixture of triterpene saponin glycosides known as escin. The objective of this study was to develop and validate a simple and fast HPLC method for quantification of beta-escin in A. hippocastanum L. hydroalcoholic extract. It was proposed, developed and validated a method by liquid chromatography with UV detection (HPLC-UV). The method was validated for specificity, linearity, limits of detection and quantification, precision, accuracy and robustness and was proven adequate for quality control of this natural product.

• 出版日期2013-9