Phytochemical analysis and Enzyme Inhibition Assay of Aerva javanica for Ulcer

作者：Khan Abdul Wajid; Jan Saleem; Parveen Shaista; Khan Rahmat Ali^*; Saeed Asma; Tanveer Abdul Jabbar; Shad Anwar Ali

来源：Chemistry Central Journal, 2012, 6(1): 76.

DOI：10.1186/1752-153X-6-76

• Aerva javanica
• Urease activity
• Ethyl acetate fraction
• Solvent-solvent extraction

Background: Aerva javanica (Burm. f.) Juss. ex Schult. (Amaranthacea) is traditionally used for the treatment of wound healings, cough, diarrhoea, ulcer and hyperglycaemia. The current study was aimed to fractionate and isolate bioactive compounds and ultimately to evaluate their anti-ulcereogenic potential.
Results: In order to achieve these aims, the fractionation, purifications and then biological potential determination of the isolated compounds was carried out. For purification purpose, initially extraction of the plant material was done with aqueous MeOH in the order of increasing polarity by using solvent-solvent extraction method. Phytochemical analysis revealed the presence of three compounds, 3-hydroxy-4 methoxybenzaldehyde (1), ursolic acid (2) and (E)-N-(4-hydroxy-3-methoxyphenethyl)-3-(4-hydroxy-3-ethoxyphenyl) acryl amide (3). Inhibition of urease activity of various fractions revealed that ethyl acetate fraction showed significant activity (P <0.05) as compared to other fractions. (E)-N-(4-hydroxy-3-methoxyphenethyl)-3-(4-hydroxy-3-ethoxyphenyl) acryl amide (3) showed marked anti ulcer activity (P <0.05).
Conclusion: These results suggested the mild potential of A. javanica against ulcer.

• 出版日期2012-7-31