Alum Adjuvant Enhances Protection against Respiratory Syncytial Virus but Exacerbates Pulmonary Inflammation by Modulating Multiple Innate and Adaptive Immune Cells

作者：Kim Ki Hye; Lee Young Tae; Hwang Hye Suk; Kwon Young Man; Jung Yu Jin; Lee Youri; Lee Jong Seok; Lee Yu Na; Park Soojin; Kang Sang Moo^*

来源：PLos One, 2015, 10(10): e0139916.

DOI：10.1371/journal.pone.0139916

Respiratory syncytial virus (RSV) is well-known for inducing vaccine-enhanced respiratory disease after vaccination of young children with formalin-inactivated RSV (FI-RSV) in alum formulation. Here, we investigated alum adjuvant effects on protection and disease after FI-RSV immunization with or without alum in comparison with live RSV reinfections. Despite viral clearance, live RSV reinfections caused weight loss and substantial pulmonary inflammation probably due to high levels of RSV specific IFN-gamma(+) IL4(-), IFN-gamma-TNF-alpha(+), IFN-gamma(+) TNF-a-effector CD4 and CD8 T cells. Alum adjuvant significantly improved protection as evidenced by effective viral clearance compared to unadjuvanted FI-RSV. However, in contrast to unadjuvanted FI-RSV, alum-adjuvanted FI-RSV (FI-RSV-A) induced severe vaccine-enhanced RSV disease including weight loss, eosinophilia, and lung histopathology. Alum adjuvant in the FI-RSV-A was found to be mainly responsible for inducing high levels of RSV-specific IFN-gamma-IL4(+), IFN-gamma-TNF-alpha(+) CD4(+) T cells, and proinflammatory cytokines IL-6 and IL-4 as well as B220(+) plasmacytoid and CD4(+) dendritic cells, and inhibiting the induction of IFN-gamma(+) CD8 T cells. This study suggests that alum adjuvant in FI-RSV vaccines increases immunogenicity and viral clearance but also induces atypical T helper CD4(+) T cells and multiple inflammatory dendritic cell subsets responsible for vaccine-enhanced severe RSV disease.

• 出版日期2015-10-15