A Potential Profibrogenic Role of Biliary Epithelium-Derived Cardiotrophin-1 in Pediatric Cholestatic Liver Disease

作者：Hua, Xiangwei; Shan, Yuhua; Li, Dawei; Xu, Dongwei; Zhang, Jiang; Yang, Taihua; Han, Longzhi; Shen, Conghuan; Xia, Yun; Chen, Qimin; Ma, Xiong; Zhang, Jianjun; Xia, Qiang^*

来源：Journal of Interferon and Cytokine Research, 2015, 35(8): 606-612.

DOI：10.1089/jir.2014.0128

• INHIBITORY FACTOR-RECEPTOR
• METABOLIC SYNDROME
• STELLATE CELLS
• HEART-FAILURE
• HYPERTROPHY
• EXPRESSION
• PROTECTION
• INJURY
• MICE
• CARDIOMYOCYTES

As a cytokine of the interleukin-6 family, cardiotrophin-1 (CT-1) has been shown to be an important endogenous protector in liver injury. Our study aimed to investigate the role of CT-1 in liver fibrosis in pediatric cholestatic liver disease (PCLD). CT-1 mRNA and protein expression levels were upregulated in PCLD liver biopsy tissues compared with controls. Immunohistochemistry and confocal microscopy of liver sections showed that CT-1 was predominantly expressed by biliary epithelium cells. Serum CT-1 was elevated significantly in the children with PCLD compared with controls. Serum CT-1 levels exhibited a moderate positive correlation with the Scheuer stage of hepatic fibrosis and serum TB levels and a weak correlation with serum ALP levels. In vitro analysis indicated that LX-2 cells preconditioned with CT-1 exhibited significant increments in proliferation and accumulation of extracellular matrix components, while also positively regulating the STAT3 and p38MAPK pathways. In conclusion, biliary epithelium-derived CT-1 may exert a profibrogenic potential in PCLD.

• 出版日期2015-8-1
• 单位上海交通大学