UV-Stressed Daphnia pulex Increase Fitness through Uptake of Vitamin D-3

作者：Connelly Sandra J^*; Walling Kelly; Wilbert Steven A; Catlin Diane M; Monaghan Cailin E; Hlynchuk Sofiya; Meehl Pamela G; Resch Lauren N; Carrera J Valerie; Bowles Stephanie M; Clark Michael D; Tan Loraine T; Cody Jeremy A

来源：PLos One, 2015, 10(7): e0131847.

DOI：10.1371/journal.pone.0131847

Ultraviolet radiation is known to be highly variable in aquatic ecosystems. It has been suggested that UV-exposed organisms may demonstrate enough phenotypic plasticity to maintain the relative fitness of natural populations. Our long-term objective is to determine the potential photoprotective effect of vitamin D-3 on Daphnia pulex exposed to acute or chronic UV radiation. Herein we report our initial findings in this endeavor. D. pulex survival and reproduction (fitness) was monitored for 5 d as a proof of concept study. Significantly higher fitness was observed in the D. pulex with D-3 than those without (most extreme effects observed were 0% survival in the absence of D-3 and 100% with 10 ppm D-3). Vitamin D-3 was isolated from the culture media, the algal food (Pseudokirchneriella), and the D. pulex and quantified using high performance liquid chromatography (HPLC). Vitamin D-3 was fluorescently labeled using a phenothiazinium dye and added to cultures of D. pulex. Images demonstrating the uptake of D-3 into the tissues and carapace of the D. pulex were acquired. Our initial findings suggest a positive role for D-3 in ecosystems as both UV-stressed algae and Daphnia sequester D-3, and D. pulex demonstrate increased fitness in the presence of D-3.

• 出版日期2015-7-6