A systematic review of p16/Ki-67 immuno-testing for triage of low grade cervical cytology

作者：Kisser A^*; Zechmeister Koss I

来源：BJOG: An International Journal of Obstetrics and Gynaecology , 2015, 122(1): 64-70.

DOI：10.1111/1471-0528.13076

• Cervical cancer screening
• human papillomavirus
• molecular diagnosis and prognosis
• p16INK4A
• Ki-67

Background Screening for cervical cancer precursors by Papanicolaou cytology is a public health success story; however, its low sensitivity entails unnecessary referrals to colposcopy of healthy women with equivocal (ASCUS) or mild dysplasia (LSIL) cytology.
Objective We assessed the accuracy of p16/Ki-67 immuno-testing for triage of low grade cervical cytology.
Search strategy We systematically searched Medline, Embase, CRD and Cochrane databases, and handsearched key references.
Selection criteria Eligible studies included women with ASCUS or LSIL cervical cytology who had undergone p16/Ki-67 testing and subsequent verification by colposcopy-directed biopsies and histologic analysis.
Data collection and analysis We extracted data on patient characteristics and test conduct, diagnostic accuracy measures and assessed the methodological quality of the studies. R software was used to perform a bivariate analysis of test performance data.
Main results Five eligible studies were identified. Four of the studies had high risk of bias. In the LSIL subgroup, the sensitivity of p16/Ki-67 testing ranged from 0.86 to 0.98, compared with 0.92-0.96 of high-risk HPV testing (hrHPV); specificity ranged from 0.43 to 0.68 versus 0.19 to 0.37, respectively. In the ASCUS subgroup, sensitivity ranged from 0.64 to 0.92 (p16/Ki67 test) versus 0.91 to 0.97 (hrHPV); specificity ranged from 0.53 to 0.81 versus 0.26 to 0.44, respectively.
Authors' conclusions p16/Ki-67 testing cannot be recommended for triage women with ASCUS or LSIL cytology due to insufficient high-quality evidence. Further studies on test performance and the impact of p16/Ki-67-based triage on health outcomes are needed for a definitive evaluation of its clinical utility.

• 出版日期2015-1