An evaluation of the utility of the hepatic differentiation method using hollow fiber/organoid culture for the development of a hybrid artificial liver device

作者：Amimoto Naoki; Mizumoto Hiroshi; Nakazawa Kohji; Ijima Hiroyuki; Funatsu Kazumori; Kajiwara Toshihisa^*

来源：Biochemical Engineering Journal, 2011, 56(1-2): 69-74.

DOI：10.1016/j.bej.2011.05.010

• Embryonic stem cells
• Hepatic differentiation
• Three dimensional culture
• Hollow fiber
• Hybrid artificial liver

To put the hybrid artificial liver (HAL) using cultured hepatocytes into practical use, it is necessary to develop a high-performance artificial liver device. We developed a novel hollow fiber (HF)/organoid culture method to induce the differentiation of pluripotent stem cells into hepatocytes. In this study, we compared the results of the hepatic differentiation using the HF/organoid culture with those using monolayer culture to evaluate its utility as a hepatic differentiation method. In both cell cultures. ES cells showed high proliferative activity immediately after cell seeding. The up-regulation of hepatocyte-specific markers such as albumin (ALB), carbamoyl phosphate s ynthetase 1 (CPS-1) and tryptophan 2,3-dioxygenase (TDO) were observed as the culture progressed, and the expression of liver-specific functions such as the removal of ammonia and albumin secretion were detected after about 2 weeks of the hepatic differentiation induction in the HF/organoid culture. However, the results were not observed in the monolayer culture. In conclusion, the HF/organoid culture method has promise as an effective tool for the differentiation of ES cells into hepatocytes.

• 出版日期2011-9-15