AlCl3 induces lymphocyte apoptosis in rats through the mitochondria-caspase dependent pathway

作者：Li, Miao; Song, Miao; Ren, Li-Min; Xiu, Chun-Yu; Liu, Jian-Yu; Zhu, Yan-zhu; Li, Yan-Fei^*

来源：Environmental Toxicology, 2016, 31(4): 385-394.

DOI：10.1002/tox.22051

• aluminum
• apoptosis
• rat spleen lymphocytes
• mitochondria
• caspase

To investigate apoptosis mechanisms in lymphocytes induced by aluminum trichloride (AlCl3) through the mitochondria-caspase dependent pathway, the spleen lymphocytes of rats were cultured with RPMI-1640 medium and exposed to AlCl(3)6H(2)O in the final concentrations of 0 (control group, CG), 0.3 (low-dose group, LG), 0.6 (mid-dose group, MG), and 1.2 (high-dose group, HG) mmolL(-1) for 24 h, respectively. Mitochondrial transmembrane potential (m), cytochrome C (Cyt C) protein expression in cytoplasm, Caspase-3 and Caspase-9 activity, Bcl-2, Bax, Caspase-3 and Caspase-9 mRNA expressions, DNA ladder and lymphocytes apoptosis index were detected. The results showed that Cyt C protein expression in cytoplasm, Caspase-3 and Caspase-9 activity, Bcl-2, Bax, Caspase-3 and Caspase-9 mRNA expressions, the ratio of Bcl-2 and Bax mRNA expression, lymphocytes apoptosis index increased, while m decreased in the AlCl3-treated groups compared with those in CG. The results indicate that AlCl3 induces lymphocyte apoptosis in rats through the mitochondria-caspase dependent pathway.

• 出版日期2016-4
• 单位东北农业大学; 中国农业科学院