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摘要
This paper describes a duplexed immunoassay method based on a functional microbeads (mu beads) array. For selective detection of dual analytes, the mu beads were conjugated with a responsive biopolymer, characterized by a different phase transition temperature. Then, the mu beads modified with elastin-like polypeptides (ELPs) were spatially localized in a single microwell through sequential injection with a buffer. Prostate specific antigen (PSA) and mouse immunoglobulin G (IgG) were selected to show the feasibility of duplexed immunoassay. The detection limit of the mu bead array with PSA and mouse IgG was 0.05 ng/mL and 0.1 ng/mL, respectively.
- 出版日期2011-6-20
