A novel room temperature nucleic acid detection method based on immobilization of adenosine-based molecular beacon

作者：Du, Ting-E.; Mao, Xun^*; Jin, Manyu; Zhang, Tian; Zhang, Yi

来源：Sensors and Actuators B: Chemical , 2014, 198: 194-200.

DOI：10.1016/j.snb.2014.03.035

• Room temperature
• Biosensor
• Fluorescence detection
• Single base mismatch discrimination

We developed a novel room temperature fluorescence DNA biosensor based on immobilization of well designed adenosine-based molecular beacon (ABMB). In the presence of coralyne, a small molecule which can react with adenosines, the ABMB would form a hairpin structure just like traditional molecular beacon used extensively. In our experiments, two kinds of adenosine-based molecular beacon probes with twelve adenosines bases at each end (A(12)-MB1-A(12) and A(12)-MB2-A(12)), and their complementary target DNA, non-complementary target DNA and single base mismatched DNAs were used as models to demonstrate the proof-of-concept. Under optimized conditions, the detection limit was estimated to be 50 pM based on S/N = 3. The ability of our developed biosensor for discriminating the single base mismatched DNA related to a human genetic disease, hereditary tyrosinemia type I, was improved undoubtedly once A12-MB2-A12 was used as capture probe comparing with previous report. It is worth to mention that the whole assay procedures were conducted under room temperature.

• 出版日期2014-7
• 单位西北大学