DODECA SATELLITE - A CONSERVED G+C-RICH SATELLITE FROM THE CENTROMERIC HETEROCHROMATIN OF DROSOPHILA-MELANOGASTER

作者：ABAD JP; CARMENA M; BAARS S; SAUNDERS RDC; GLOVER DM; LUDENA P; SENTIS C; TYLERSMITH C; VILLASANTE A

来源：Proceedings of the National Academy of Sciences of the United States of America, 1992, 89(10): 4663-4667.

DOI：10.1073/pnas.89.10.4663

• CENTROMERIC SATELLITE, DROSOPHILA YEAST ARTIFICIAL CHROMOSOME LIBRARY
• INSITU HYBRIDIZATION

To identify sequences from the centromeric region, we have constructed a Drosophila melanogaster yeast artificial chromosome (YAC) library and screened it with purified DNA from the minichromosome Dp(1;f)1187 derived from the X chromosome. We describe the structure of one clone isolated in this way. This YAC is structurally unstable and contains tandemly repeated G + C-rich 11-mer and 12-mer units, which we call dodeca satellite. Most of this satellite is located near the centromere of an autosome. Cross-hybridizing sequences are found in the genomes of organisms as distant as Arabidopsis thaliana and Homo sapiens.

• 出版日期1992-5-15