New Drug Screening Model Using Enzymes Immobilized on Mesoporous Materials: A Proof-of-Concept Study Using Immobilized α-Glucosidase and Acarbose

作者：Hu, Yinghui; An, Jing; Liu, Xiujie; Wang, Huiheng; Dai, Rongji^*; Deng, Yulin

来源：Journal of Nanoscience and Nanotechnology, 2016, 16(12): 12460-12469.

DOI：10.1166/jnn.2016.12692

• Mesoporous Materials
• Immobilized alpha-Glucosidase
• Drug Screening

Enzyme immobilization increases the availability of the enzyme to the substrate with a higher turnover over a considerable period. For this purpose, a variety of mesoporous materials with different diameters were synthesized by various methods using different ratios of P123 (template agent) to 1,3,5-trimethylbenzene (expanding agent). These versatile materials were then characterized by transmission electron microscopy and N-2 adsorption-desorption analysis. alpha-Glucosidase was successfully immobilized on all the synthesized materials, but the P123/TMB = 4/3-COOH-PMO material had a higher loading rate and enzyme activity. Furthermore, applications of this material were best performed in a column to immobilize the enzymes. Additionally, the synthesized material was further tested using acarbose as a model compound for drug screening. The immobilized alpha-glucosidase was packed into a column and connected to HPLC instrument to screen 20 small molecular compounds. Using this method, several drugs that might strongly inhibit alpha-glucosidase were identified. Therefore, this method can be further used in drug screening for chemical drugs and traditional Chinese medicines to expedite new drug research.

• 出版日期2016-12
• 单位北京理工大学