Agonist mobility on supported lipid bilayers affects Fas mediated death response

作者：Sanchez M Florencia; Levi Valeria; Weidemann Thomas^*; Carrer Dolores C

来源：FEBS LETTERS, 2015, 589(23): 3527-3533.

DOI：10.1016/j.febslet.2015.10.009

• Fluorescence correlation spectroscopy
• Supported lipid bilayer
• Apoptosis
• Functionalized surface
• Fas receptor

Extrinsic apoptosis is initiated by recognition and clustering of the single-pass transmembrane proteins Fas ligand and Fas expressed at the surface of closely apposed lymphocytes and target cells, respectively. Since Fas-mediated death response was mainly studied with soluble antibodies, the mobility constraints for receptor activation by a membrane embedded agonist is not well understood. We explored this influence by stimulating apoptosis on functionalized supported lipid bilayers, where we quantified agonist mobility by z-scan fluorescence correlation spectroscopy. Using different lipid compositions, we show that the apoptotic response correlates with increased lateral mobility of the agonist in the lipid bilayer.

• 出版日期2015-11-30