科研之友
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摘要
Transgenic mice are highly valuable tools for biological research as they allow cell type-specific expression of functionally instrumental genes. In this protocol, the generation of bacterial artificial chromosome (BAG) transgenic constructs is described. We give an overview of different transgenic inserts, such as fluorescent proteins (alone or in combination with Cre variants), diphtheria toxin receptor, lacZ, and light-activated ion channels. The most reliable and versatile approach to express these genes is by using BACs, which allow "highjacking" of the expression pattern of a gene without characterizing its transcriptional control elements. Here, we describe the necessary cloning techniques compared with conventional transgenesis. With the provided "toolbox" of already available transgene constructs, the generation of the BAG transgenes is made easy and rapid. We provide a comprehensive outline how to insert the different transgenes into a chosen BAG by either ET cloning or recombineering. We also describe in detail the methods to identify the correct insertion and the integrity of the final BAG construct, and finally, the preparation of the BAG DNA for oocyte injection is described. genesis 48:264-280, 2010.
- 出版日期2010-4
