A statistically inferred microRNA network identifies breast cancer target miR-940 as an actin cytoskeleton regulator

作者：Bhajun Ricky; Guyon Laurent; Pitaval Amandine; Sulpice Eric; Combe Stephanie; Obeid Patricia; Haguet Vincent; Ghorbel Itebeddine; Lajaunie Christian; Gidrol Xavier^*

来源：Scientific Reports, 2015, 5(1): 8336.

DOI：10.1038/srep08336

MiRNAs are key regulators of gene expression. By binding to many genes, they create a complex network of gene co-regulation. Here, using a network-based approach, we identified miRNA hub groups by their close connections and common targets. In one cluster containing three miRNAs, miR-612, miR-661 and miR-940, the annotated functions of the co-regulated genes suggested a role in small GTPase signalling. Although the three members of this cluster targeted the same subset of predicted genes, we showed that their overexpression impacted cell fates differently. miR-661 demonstrated enhanced phosphorylation of myosin II and an increase in cell invasion, indicating a possible oncogenic miRNA. On the contrary, miR-612 and miR-940 inhibit phosphorylation of myosin II and cell invasion. Finally, expression profiling in human breast tissues showed that miR-940 was consistently downregulated in breast cancer tissues

• 出版日期2015-2-12
• 单位Mines Paristech; 中国地震局