Mild gut inflammation modulates the proteome of intestinal Escherichia coli

作者：Schumann Sara; Alpert Carl; Engst Wolfram; Klopfleisch Robert; Loh Gunnar; Bleich Andre; Blaut Michael^*

来源：Environmental Microbiology, 2014, 16(9): 2966-2979.

DOI：10.1111/1462-2920.12192

Using interleukin 10-deficient (IL-10-/-) and wild-type mice monoassociated with either the adherent-invasive Escherichia coliUNC or the probiotic E.coliNissle, the effect of a mild intestinal inflammation on the bacterial proteome was studied. Within 8 weeks, IL-10-/- mice monoassociated with E.coliUNC exhibited an increased expression of several proinflammatory markers in caecal mucosa. Escherichia coliNissle-associated IL-10-/- mice did not do so. As observed previously for E.coli from mice with acute colitis, glycolytic enzymes were downregulated in intestinal E.coliUNC from IL-10-/- mice. In addition, the inhibitor of vertebrate C-type lysozyme, Ivy, was upregulated on messenger RNA (mRNA) and protein level in E.coliNissle from IL-10-/- mice compared with E.coliUNC from these mice. Higher expression of Ivy in E.coliNissle correlated with an improved growth of this probiotic strain in the presence of lysozyme-ethylenediaminetetraacetic acid (EDTA). By overexpressing Ivy, we demonstrated that Ivy contributes to a higher lysozyme resistance of E.coli, supporting the role of Ivy as a potential fitness factor. However, deletion of Ivy did not alter the growth phenotype of E.coliNissle in the presence of lysozyme-EDTA, suggesting the existence of additional lysozyme inhibitors that can take over the function of Ivy.

• 出版日期2014-9