alpha SMA-Expressing Perivascular Cells Represent Dental Pulp Progenitors In Vivo

作者：Vidovic, I.; Banerjee, A.; Fatahi, R.; Matthews, B. G.; Dyment, N. A.; Kalajzic, I.; Mina, M.^*

来源：Journal of Dental Research, 2017, 96(3): 323-330.

DOI：10.1177/0022034516678208

• odontoblasts
• pulp biology
• dentinogenesis
• reparative dentin
• stem cells
• dentin sialophosphoprotein

The goal of this study was to examine the contribution of perivascular cells to odontoblasts during the development, growth, and repair of dentin using mouse molars as a model. We used an inducible, Cre-loxP in vivo fate-mapping approach to examine the contributions of the descendants of cells expressing the alpha SMA-CreERT2 transgene to the odontoblast lineage. In vivo lineage-tracing experiments in molars showed the contribution of alpha SMA-tdTomato(+) cells to a small number of newly formed odontoblasts during primary dentinogenesis. Using an experimental pulp exposure model in molars to induce reparative dentinogenesis, we demonstrate the contribution of alpha SMA-tdTomato(+) cells to cells secreting reparative dentin. Our results demonstrate that alpha SMA-tdTomato(+) cells differentiated into Col2.3-GFP(+) cells composed of both Dspp(+) odontoblasts and Bsp+ osteoblasts. Our findings identify a population of mesenchymal progenitor cells capable of giving rise to a second generation of odontoblasts during reparative dentinogenesis. This population also makes a small contribution to odontoblasts during primary dentinogenesis.

• 出版日期2017-3