Apoptosis-like cell death induced by Salmonella in Acanthamoeba rhysodes

作者：Feng, Ye; Hsiao, Yi-Hsing; Chen, Hsiu-Ling; Chu, Chishih; Tang, Petrus; Chiu, Cheng-Hsun^*

来源：Genomics, 2009, 94(2): 132-137.

DOI：10.1016/j.ygeno.2009.05.004

• Salmonella
• Acanthamoeba rhysodes
• Cell death
• Microarray

Free-living amoebae act as environmental hosts of several intracellular pathogens. We examined the interaction between Acanthamoeba rhysodes and Salmonella, a human intracellular pathogen. There was no difference among three different serovars of Salmonella in terms of their growth within A. rhysodes over time. The number of intracellular bacteria increased at 6 h post-infection, and the viability of A. rhysodes was significantly reduced at 24 h post-infection. Amoebic cell death was characterized by TUNEL and Annexin V assay, without DNA ladder identified, indicating an apoptosis-like cell death in Salmonella-infected A. rhysades. Global gene expression screening between intracellular and extracellular Salmonella by microarray and quantitative PCR showed that genes from Salmonella pathogenicity islands and virulence plasmid were up-regulated within A. rhysodes. The phase-dependent expression pattern suggests their distinct roles in the pathogenesis. A. rhysodes and Salmonella provide a model to study transient symbiosis between bacterial pathogens and protozoa in an aquatic ecosystem.

• 出版日期2009-8
• 单位浙江大学; 中国科学院北京基因组研究所; 长春大学