A rapid fluorescence assay for hSMUG1 activity based on modified molecular beacon

作者：Yang, Xue; Tong, Chunyi; Long, Ying; Liu, Bin^*

来源：Molecular and Cellular Probes, 2011, 25(5-6): 219-221.

DOI：10.1016/j.mcp.2011.09.001

• hSMUG1
• Molecular beacon
• Real-time

A new method for assay of hSMUG1 in real-time using molecular beacon is reported. hSMUG1 could be detected linearly in the range from 0.67 nM to 10.05 nM and the detection limit is 0.168 nM. In addition, this method was applied to detect the activity of hSMUG1 in tumor cells and study kinetics. The probe with low background signal has been shown to be suitable for the real-time monitoring of hSMUG1 activity, making this a promising method of high-throughput clinical sample analysis.

• 出版日期2011-12
• 单位湖南大学