Donor-derived DNA in hair follicles of recipients after allogeneic hematopoietic stem cell transplantation

作者：Jacewicz R^*; Lewandowski K; Rupa Matysek J; Jedrzejczyk M; Brzezinski P M; Dobosz T; Jonkisz A; Szram S; Komarnicki M; Berent J

来源：Bone Marrow Transplantation, 2010, 45(11): 1638-1644.

DOI：10.1038/bmt.2010.27

• HSCT
• hair follicles chimerism
• sex-mismatched allografts
• Y-STR
• SRY

The hair follicles of recipients of allogeneic hematopoietic SCT (HSCT) constitute the tissue with the greatest need for regeneration after high-dose chemotherapy. Previous studies have shown a lack of donor-derived DNA in the hair follicles of recipients. Therefore, we carried out a study to determine whether male donor-derived genetic material can be found in female recpients' hair follicles after HSCT. Fluorescent-based PCR with analyses of Y-chromosome STR (Y-STR) and RQ-PCR with the sex-determining region Y (SRY) were used independently to evaluate chimerism status. Our results proved the existence of donor-derived stem DNA in the recipients' hair follicle cells. This report undermines the validity of data indicating that hair follicle cells maintain 100% of recipient origin. Bone Marrow Transplantation (2010) 45, 1638-1644; doi:10.1038/bmt.2010.27; published online 22 February 2010

• 出版日期2010-11