Antibacterial and DNA degradation potential of silver nanoparticles synthesized via green route

作者：Manna Dilip K; Mandal Arnit K; Sen Ipsita K; Maji Praloy K; Chakraborti Soumyananda; Chakraborty Ranadhir; Islam Syed S^*

来源：International Journal of Biological Macromolecules, 2015, 80: 455-459.

DOI：10.1016/j.ijbiomac.2015.07.028

• AgNPs-PS conjugates
• Multiple antibiotic resistant
• Antibacterial activity

Silver nanoparticles (AgNPs) were synthesized using a hetero polysaccharide (PS) isolated from Lentinus squarrosulus (Mont.) Singer. The polysaccharide fraction (consisting of glucose, fucose and galactose) serves the role of both reducing as well as stabilizing agent. UV-vis spectroscopy showed maximum absorbance at 407 nm due to surface plasmon resonance. High resolution transmission electron microscopy (HRTEM) exhibited that the average diameter of the nanoparticles was 2.78 +/- 1.47 nm. The XRD analysis revealed face-centered cubic (fcc) geometry of silver nanoparticles. Antibacterial activity of the AgNPs PS conjugate was tested against multiple antibiotics resistant (MAR) Escherichia coli strain MREC33 and found that the killing was due to generation of reactive oxygen species (ROS). Internalization of AgNPs PS conjugate along with its DNA degradation capability was demonstrated using flow cytometry. AgNPs PS conjugates showed negligible toxicity to human RBCs. This LD50 dosage of AgNPs-PS conjugates in combination with each of the four antibiotics (ampicillin, azithromycin, kanamycin and netilmicin) to which E. coil MREC33 was resistant, showed synergistic effect to inhibit complete bacterial growth.

• 出版日期2015-9