A Genetic Reporter System to Gauge Cell Proliferation Rate

作者：Peacock Ryan W S; Wang Clifford L^*

来源：Biotechnology and Bioengineering, 2011, 108(9): 2003-2010.

DOI：10.1002/bit.23163

• genetic reporter
• proliferation rate
• E2F
• cell cycle

In higher eukaryotes, E2F transcription factors often drive expression of genes necessary for the cell cycle, notably the G1/S phase transition. With conventional transcriptional reporter systems, expression of a reporter gene from an E2F-responsive promoter would allow one to identify the fraction of cells making this transition. Here, we have engineered an E2F-responsive genetic reporter system that outputs the proliferation rate. The system takes advantage of the long half-lives of fluorescent protein reporters and output signal normalization. By doing so, it converts dynamic pulses of E2F activity into an analog output proportional to the proliferation rate. Such a system should be useful for applications involving high-throughput drug or genetic screens, investigation of cellular environment, and biological engineering. Biotechnol. Bioeng. 2011; 108: 2003-2010.

• 出版日期2011-9