Adaptation of Ti(III)-NTA colorimetric assay for use in detecting microbial demethylation of lignin and lignin derived compounds in aerobic conditions

作者：Gibson A^*; Dekker R F H; Malek L

来源：Journal of Microbiological Methods, 2014, 101: 28-32.

DOI：10.1016/j.mimet.2014.03.007

• Lignin-derived compound
• Demethylation
• Ti(III)-NTA

An anaerobic colorimetric assay for quantifying microbial demethylation activity was adapted for aerobic use in studying lignin and lignin-derived compounds. Standard curves of 0-500 mu M pyrocatechol with and without 0.3% lignin demonstrated the use in either case. This method detects demethylation products up to 500 mu M without using additional dilutions.

• 出版日期2014-6