NF-kappa B activation is cell type-specific in the heart

作者：Rivera Serrano Efrain E; Sherry Barbara^*

来源：Virology, 2017, 502: 133-143.

DOI：10.1016/j.virol.2016.12.022

• NF-kappa B
• Myocarditis
• Cardiac myocyte
• Cardiac fibroblast
• Cardiomyocyte
• Golgi
• Reovirus
• Heart

Viral myocarditis is common and can progress to cardiac failure. Cardiac cell pro-inflammatory responses are critical for viral clearance, however sustained inflammatory responses contribute to cardiac damage. The transcription factor NF-kappa B regulates expression of many pro-inflammatory cytokines, but basal and induced activation of NF-kappa B in different cardiac cell types have not been compared. Here, we used primary cultures of cardiac myocytes and cardiac fibroblasts to identify cardiac cell type-specific events. We show that while viral infection readily stimulates activation of NF-kappa B in cardiac fibroblasts, cardiac myocytes are largely recalcitrant to activation of NF-kappa B. Moreover, we show that cardiac myocyte subpopulations differ in their NF-kappa B subcellular localization and identify the cis-Golgi as a cardiac myocyte-specific host compartment. Together, results indicate that NF-kappa B-dependent signaling in the heart is cardiac cell type-specific, likely reflecting mechanisms that have evolved to balance responses that can be either protective or damaging to the heart.

• 出版日期2017-2