Northern blot detection of endogenous small RNAs (similar to 14 nt) in bacterial total RNA extracts

作者：Beckmann Benedikt M; Gruenweller Arnold; Weber Michael H W; Hartmann Roland K^*

来源：Nucleic Acids Research, 2010, 38(14): e147.

DOI：10.1093/nar/gkq437

Here we describe a northern blot procedure that allows the detection of endogenous RNAs as small as similar to 14 nt in total RNA extracts from bacteria. RNAs that small and as part of total bacterial RNA extracts usually escape detection by northern blotting. The approach combines LNA probes 5'-digoxigenin-endlabeled for non-radioactive probe detection with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide-mediated chemical crosslinking of RNAs to nylon membranes, and necessitates the use of native PAGE either with the TBE or MOPS buffer system.

• 出版日期2010-8