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摘要
Astrocytes exhibit dynamic Ca2 mobilization, such as Ca2 wave and Ca2 oscillation, via an inositol 1,4,5-triphosphate-induced Ca2 release (IICR)-dependent mechanism. The physiological functions of astrocytic Ca2 mobilization, however, are poorly understood. To investigate this issue, we created a plasmid encoding an enhanced green fluorescent protein-tagged inositol 1,4,5 -triphosphate absorbent protein and expressed it in cultured astrocytes. Expression of this protein inhibited both IICR and the Ca2 wave in cultured astrocytes. By combining this method to the single cell electroporation technique, we were able to inhibit IICR specifically in astrocytes in an astrocyte-neuron co-culture system. Our approach provides a useful tool for direct examination of the physiological role of astrocytic Ca2 signaling on neuronal function.
