Acetylcholinesterase biosensor based on the mesoporous carbon/ferroferric oxide modified electrode for detecting organophosphorus pesticides

作者：Zhang, Qianqian; Xu, Qingcui; Guo, Yemin^*; Sun, Xia^*; Wang, Xiangyou

来源：RSC Advances, 2016, 6(29): 24698-24703.

DOI：10.1039/c5ra21799g

• GLUCOSE BIOSENSOR
• CARBON NANOTUBE
• NANOCOMPOSITE
• MATRIX
• FILM
• TETRATHIAFULVALENE
• IMMOBILIZATION
• SPECTROMETRY
• CARBARYL
• ENZYME

In this paper a biosensor modified by ordered mesoporous carbon-chitosan (OMC-CS)/ferroferric eoxide-chitosan (Fe3O4-CS) was developed on the surface of screen-printed carbon electrodes (SPCEs). The acetylcholinesterase (AChE) was modified onto the film to prepare an AChE biosensor. Chitosan was used as a dispersant to disperse OMC and Fe3O4. The OMC and Fe3O4 were used to enhance the electrochemical response. Before the detection of organophosphorus (OP) pesticides, the electrochemical behaviour of AChE/OMC-CS/Fe3O4-CS/SPCE was studied with cyclic voltammetry, and the results showed that the chitosan can disperse OMC and Fe3O4 evenly and fix them on the electrode surface firmly. OMC and Fe3O4 have a significant synergistic effect towards electron transfer. The parameters affecting performance, such as the pH of the test solution, the amount of AChE and the time of inhibition have been optimized. Under optimum conditions, using methamidophos and chlorpyrifos as model compounds, this biosensor showed a wide range, low detection limit, good reproducibility and high stability. Moreover, the AChE/OMC-CS/Fe3O4-CS/SPCE biosensor can be used for the detection of real samples, and is suitable for field testing of OP pesticide residues.

• 出版日期2016
• 单位山东理工大学